The answer lies in The very fact that there are a number of isoforms of each troponin subunit which happen to be encoded by unique genes, many of which might be expressed selectively in cardiac muscle. Although TnI and TnT have distinctive cardiac and skeletal isoforms, they share a typical isoform of TnC: the slow-twitch skeletal muscle isoform (s
The distinctions in AA sequences permitted growth of quantitative assays for cTnI/T. Most cTn assays are non-aggressive enzyme-connected immunosorbent assays (ELISA) based upon the sandwich basic principle, using the large specificity and affinity of antibodies.31 The assay is predicated with a capture antibody which binds to a particular epitope o
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